Searchable Database of Pan-Gene Family Members Involved in Ascorbic Acid Metabolism

L-抗坏血酸(AsA),又称为维生素C,在植物生长发育过程中扮演者重要的角色。AsA作为一种重要的抗氧化剂,不仅能维持氧化还原平衡,抵抗生物和非生物胁迫,而且通过复杂的信号转导网络调节植物生长、诱导开花和延缓衰老。AsA代谢是一个较为复杂的生理过程,涉及一系列功能基因,在多个物种上系统鉴定AsA代谢相关的基因,对于揭示AsA在生物体内的富集机制以及通过基因工程技术提高动植物体内AsA含量具有重要意义。

为了满足科研人员对这些基因信息的需求,减少AsA代谢相关基因的鉴定工作,避免基因命名混乱,构建了“抗坏血酸代谢基因泛基因家族成员检索数据库”。本网站对21个物种(拟南芥、番茄、葡萄、中华猕猴桃‘红阳’、中华猕猴桃‘Red5’、中华猕猴桃‘东红’、野生毛花猕猴桃、毛花猕猴桃‘华特’、毛花猕猴桃‘赣绿1号’、山梨猕猴桃、阔叶猕猴桃、苹果、梨、桃、刺梨‘贵农5号’、枣、甜橙、晚白柚、椪柑、枳壳和枸橼)的AsA代谢基因(17个)的基因成员家族进行鉴定。每一个基因的基因家族成员都按染色体上出现顺序进行命名,并且具有专属的子页面;科研人员获取该基因在21个物种上的同源基因,并在主页面上点击“Download”直接下载这21个物种的所有蛋白序列。

L-ascorbic acid (AsA), also known as vitamin C, plays a crucial role in plant growth and development. As an important antioxidant, AsA not only helps maintain redox balance and resist both biotic and abiotic stresses, but also regulates plant growth, induces flowering, and delays senescence through complex signal transduction networks. AsA metabolism is a complex physiological process involving a series of functional genes. Systematic identification of genes related to AsA metabolism across multiple species is of great significance for understanding the mechanisms underlying AsA accumulation in organisms and for enhancing AsA content in plants and animals through genetic engineering.

To meet the needs of researchers for information on these genes, reduce the workload of identifying AsA-related genes, and avoid confusion in gene nomenclature, we have developed the “Ascorbic Acid Metabolic Gene Homolog Retrieval Database.” This website identifies the gene family members of 17 AsA metabolic genes across 21 species, including Arabidopsis thaliana, tomato, grape, Actinidia chinensis cv ‘Hongyang’, cv ‘Red5’, and cv ‘Donghong’, wild Actinidia eriantha, A. eriantha cv ‘White’ and cv ‘Ganlv 1’, A. latifolia, A.rufa, apple, pear, peach, Rosa roxburghii ‘Guinong 5’, jujube, sweet orange, pomelo, ponkan, trifoliate orange, and citron. Each gene family member is named according to its order on the chromosome and has a dedicated subpage. Researchers can obtain homologous genes of a given AsA metabolic gene across the 21 species and directly download the corresponding protein sequences by clicking “Download” on the main page.

Ⅰ   The L-galactose pathway : PMI,PMM,GMP,GME,GGP,GPP
Ⅱ   The D-galacturonic acid pathway : PME,GaIUR
Ⅲ  The inositol pathway :MIOX
Ⅳ  The L-gulose pathway :Has not been identified yet!
Ⅴ   The cyclic regeneration pathways :MDHAR,DHAR,AO/APX
Ⅵ  The degradation pathway:GaIDH,GalLDH

ASA代谢示意图

网站文献(Website literature)